Наукові праці співробітників кафедри біотехнології

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Aclinic method of trap culture for arbuscular mycorrhizal fungi
(Polish Mycological Society, Warsaw, 2019) Drazhnikova, Anna; Andrianova, Tetiana; Дражнікова, Анна Вікторівна; Андріанова, Тетяна Володимирівна
Analysis of some aspects of microorganisms application for inorganic materials treatment
(Національний авіаційний університет, 2014-07-20) Карпенко, Валерій Іванович; Лазарєв, Віктор Георгійович; Дражнікова, Анна Вікторівна
The article discusses the possibility of integrating multiple methods of biotechnology, namely the elimination of oil pollution using biodegradation, biosorption of heavy metals, obtaining of microbial nanoparticles, technology of microbial fuel cells and others. Authors defined conditions and features of their possible integrated application.
Antibiotic resistance of Escherichia coli isolated from Ukrainian Betula verrucosa Ehrh. pollen after microbiological analysis
(Slovak Agricultural University in Nitra, 2013-08-01) Shevtsova, Tetiana; Hleba, Lukáš; Kačániová, Miroslava; Brindza, Ján; Garkava, Kateryna; Гаркава, Катерина Григорівна
Seven samples of silver birch pollen from different habitat of Ukraine were investigated in order to estimate their contamination with the Enterobacteriaceae family, anaerobic bacteria and fungi. Also resistance of 108 strains of Escherichia coli isolated from seven samples of Ukrainian Betula verrucosa Ehrh. pollen against 5 antibiotics: ampicillin, chloramphenicol, meropenem, ceftriaxone and ofloxacin were determined. Disc diffusion method was used for antibiotic suceptibility testing according to EUCAST 2012. It is established the concentrations of enterobacteria ranged from 0.00 to 4.16 log cfu/g, of anaerobic bacteria – 2.48 to 4.90 log cfu/g and concentration of fungi ranged from 2.48 to 4.14 log cfu/g. Degree of pollen contamination is different depending on the habitats. The resistance of E. coli isolates was determined against ampicillin, chloramphenicol, meropenem and ofloxacin. But intermediate resistance in the 33.3% of E. coli isolates and susceptibility in the 8.3% to ceftriaxone was found out. Antibiotic resistance was evaluated for all samples of pollen in whole.
Antimicrobial properties of biologically active substances of ginger root extract
(2014-09) Васильченко, Ольга Анатоліївна; Кудас, В.Є.
This work considers the investigation of biologically active substances of ginger root extract antimicrobial properties and assessing the feasibility of ginger root extract using as antimicrobial agent. Main characteristics of ginger root biologically active substances and ginger effect on human body are explained.
Application of immunoglobulin-binding proteins A, G, L in the affinity chromatography
(2014) Васильченко, Ольга Анатоліївна; Sviatenko, O.V.; Gorbatiuk, O.B.
Proteins A, G and L are native or recombinant proteins of microbial origin that bind to mammalian immunoglobulins. Preferably recombinant variants of proteins A, G, L are used in biotechnology for affinity sorbents production. Сomparative characteristics of proteins A, G, L and affinity sorbents on the basis of them, advantages and disadvantages of these proteins application as ligands in the affinity chromatography are done. Analysis of proteins A, G, L properties is presented. Binding specificities and affinities of these proteins differ between species and antibody subclass. Protein А has high affinity to human IgG1, IgG2, IgG4, mouse IgG2a, IgG2b, IgG3, goat and sheep IgG2, dog, cat, guinea pig, rabbit IgG. Protein G binds strongly to human, mouse, cow, goat, sheep and rabbit IgG. Protein L has ability of strong binding to immunoglobulin kappa-chains of human, mouse, rat and pig. Expediency of application of affinity chromatography with usage of sorbents on the basis of immobilized proteins A, G, L are shown for isolation and purification of antibodies different classes. Previously mentioned method is used as an alternative to conventional methods of protein purification, such as ion-exchange, hydrophobic interactions, metal affinity chromatography, ethanol precipitation due to simplicity in usage, possibility of one-step purification process, obtaining of proteins high level purity, multiuse at maintenance of proper storage and usage conditions. Affinity sorbents on the basis of immobilized proteins A, G, L are used not only for antibodies purification, but also for extraction of different antibodies fractions from blood serum.
Biotechnological aspects of hydrocarbons biodegradation
(2012) Васильченко, Ольга Анатоліївна; Aliieva, O.R.; Matvyeyeva, O.L.; Salata, A.M.
In review the present situation of petroleum pollution, its consequences and basic oil composition are described. The up to date survey of hydrocarbon degradation microbiology and major strains are presented with respect to following practical introduction of obtained data. A general characteristic of oil hydrocarbons biodegradation is done. Biodegradation metabolism of petroleum products different categories and its biochemistry are described. Principal biochemical pathways are indicated. Biotechnological properties of hydrocarbon degrading microorganisms including that of industrial significance are investigated. Much attention is given to biological and environmental factors that influence completeness and rate of hydrocarbons biodegradation. It was established that microorganisms consortium biodegradation is faster and more complete that single species ones. The effects of hydrocarbons presence in medium on biochemical and cytological levels of microbial metabolism are also analyzed. The major methods of microbial resistance to petroleum products toxic influence are described. It is shown that one of the most important among them is metabolic processes shifting towards surfactants.
BIOTECHNOLOGICAL ASPECTS OF POLY-β-HYDROXYBUTYRATE BIOSYNTHESIS
(2011) Vasylchenko, O. A.; Yastremskaya, L. S.; Salata, A. M.; Salata, O. S.; Fedun, N. O.
In the review polyhydroxyalkanoates characteristic, including poly β hydroxybutyrate (P3HB) which is a polymer produced by microorganisms (Ralstonia eutropha — superproducer) is done. Biochemical pathways and conditions of P3HB microbial synthesis have been examined. Thermoplastic features of microbial P3HB in comparison with classic chemical polymers (polyethylene and polypropy lene) have been analyzed. It was shown that according to its properties it was the most similar to syn thetic polypropylene in comparison to other biopolymers, and it could be its good substitute. Microbial P3HB ability for biodestruction to CO2 and H2O has shown as extremely perspective pro perty for this polymer usage in different industry branches and for environment saving against anthro pogenous pollution.
Changes in expression of BetV1 allergen of silver birch pollen in urbanized area of Ukraine
(Taylor & Francis Group, 2013) Žiarovská, Jana; Labajová, Mária; Ražná, Katarína; Bežo, Milan; Śtefunová, Veronika; Shevtsova, Tetiana; Garkava, Kateryna; Brindza, Ján
The aim of the study was to determinate the level of expression of silver birch allergen Betv1 in pollen samples from different Ukraine areas by RT-qPCR SYBR Green assay. Protocol for quantifying the expression of Betv1 allergen was developed when testing of three housekeeping genes—cyclophylin, alpha-tubulin and transcription factor CBF1. Samples from urbanized area was analysed by real-time PCR when a sample from forest growth conditions was used as a calibrator. Real-time PCR based quantifying of Bet v 1 provides a useful method for rapid and sensitive analyses of this silver birch allergen. Our results show higher expression levels in samples from central parts of urbanized area as housing estates when compared to the samples from borders of the urbanized area.
Chem. listy 108, 10801083(2014) Laboratorní přístroje a postupy 1080 antioxidačná aktivita peľu briez vo vzťahu k expresii betv1 alergénu
(2014) Гаркава, Катерина Григорівна; Žiarovská, Jana; Shevtsova, Tetiana; Gažo, Ján; Brindza, Ján; Bežo, Milan
The aim of the study was to determine the total anti-oxidant activity and expression of silver birch allergen BetV1 in pollen samples from various Ukraine areas. The obtained results show higher expression of BetV1 allergen in samples from central parts of urbanized areas (ca. 1.5 times) compared with the samples from borders of the urbanized areas (ca. 0.55 times). The total antioxidant ac-tivity of aqueous pollen extracts ranged from 80.5 % to 85.5 %, for ethanolic extracts 60.3–84.9 %. Positive corre-lation was found between antioxidant activity in ethanolic pollen extract and BetV1 allergen expresion.
Cleaning of sewages with use of pinotenk
(2016) Решетняк, Людмила Расулівна; Семенова, О.І.; Семенова, О.А.; Бублієнко, Н.О.; Шилофост, Т.
Utilization of hydrocarbons contained sewages is a difficult process, but possible as compared to cleaning of household flows. Oil products in sewer water are in the dissolved and emulsified condition. For the removal of such contaminations fraction it is expedient to use the process of biosorption before a bioscrubbing. The exception of oil products from effluents is expedient to carry out in pinotenk, that is a device with gas-liquid reflow. Processes of biosorption of contaminations and their partial oxidization by the organisms of active silt are taking place in pinotenk
Comparative analysis of sources for chitosan obtaining
(2015) Васильченко, Ольга Анатоліївна; Abramova, M.V.
The article highlights the properties and applications of biopolymers such as chitin and chitosan. Traditional sources of chitosan obtaining and problems of their application are reviewed. Alternative, more perspective sources for chitosan obtaining are proposed.
Contamination of Betula verrucosa Ehrh. pollen by microorganisms, mycotoxins and heavy metals
(Faculty of Biotechnology and Food Sciences of SAU, 2014-06-01) Shevtsova, Tetiana; Kačániová, Miroslava; Garkava, Kateryna; Brindza, Ján; Petrova, Jana; Гаркава, Катерина Григорівна
Pollen samples of silver birch from Ukraine and Slovakia were investigated in order to estimate their contamination with heavy metals (Cd, Pb, Hg, Cr, As, Ni, Se, Co), representatives of the Enterobacteriaceae family, mesophilic aerobic bacteria, fungi and mycotoxins. The study is focused on the comparative analysis among pollen samples when complex of biotic, abiotic and anthropogenic factors in different places of growth affect. The obtained results are compared to the existing requirements. It is established excess of limits on the content of cadmium, lead, chromium, the concentrations of enterobacteria and mesophilic aerobic bacteria in some samples of birch pollen. The Tukey test was used to determine the differences between the means at a level of P < 0.05. Also in this work, the steps from collection to storage of wind-pollinated pollen were analyzed.
Contamination of Betula verrucosa Ehrh. pollen by microorganisms, mycotoxins and heavy metals
(2014) Гаркава, Катерина Григорівна; Shevtsova, T.; Kačániová, M.; Brindza, J.; Petrova, J.
Pollen samples of silver birch from Ukraine and Slovakia were investigated in order to estimate their contamination with heavy metals (Cd, Pb, Hg, Cr, As, Ni, Se, Co), representatives of the Enterobacteriaceae family, mesophilic aerobic bacteria, fungi and mycotoxins. The study is focused on the comparative analysis among pollen samples when complex of biotic, abiotic and anthropogenic factors in different places of growth affect. The obtained results are compared to the existing requirements. It is established excess of limits on the content of cadmium, lead, chromium, the concentrations of enterobacteria and mesophilic aerobic bacteria in some samples of birch pollen. The Tukey test was used to determine the differences between the means at a level of P < 0.05. Also in this work, the steps from collection to storage of wind-pollinated pollen were analyzed.
EFFECT OF ALFALFA MEDICAGO SATIVA ON VITAL ACTIVITY OF YEAST S. CEREVISIAE
(2018) Решетняк, Людмила Расулівна; Семенова, О.І.; Коломоєць, Н.А.
Purpose: improvement of biotechnological qualities of baker’s yeast promotes the intensification of fermentation processes, semis maturation, and ultimately the improvement of the quality of final product. Yeasts do not always satisfy the requirements. Therefore studies that are aimed at the intensification of fermentative activity and improvement of the quality of yeasts are interest. The problem of examination of stimulating effect of substances from different nature and origin on the growth and quality of yeasts is not new. One of the methods of intensification of the technological process of yeast production is selection of culture medium by replacement of mineral source of nitrogen with plant-based source. In our opinion, it is suitable to use for this purpose. Methods: in this study, we examined the ability of yeast cells to utilize organic nitrogen from a plant-based source of protein such as alfalfa granules. The use of this method allowed us to carve out the optimal components of the nutrient medium, which has provided the yeast with improved quality indicators. Results: we examined the effect of alfalfa as a source of nitrogen on the vital activity of yeast S. cerevisiae. Discussion: Alfalfa granules can be used in a cultural medium for barm yeast cultivation on a production scale.
Effect of prebiotics on lyzosymic activity of the Bacillus genus bacteria
(2013) Васильченко, Ольга Анатоліївна; Saveka, М.A.
Beneficial effects on a host health of probiotic strains of spore-forming bacteria of the Bacillus genus in combination with prebiotic substance lactulose were investigated. The influence of prebiotic lactulose on the Bacillus subtilis 39 and Bacillus subtilis 51 lyzosymic activity were analyzed. The dependence of the growth inhibition zones extension on lactulose quantity introduced into cultural medium was displayed.
Evaluation of heating energy savings after renovation of a standard 5-storey residential building
(Metallurgical and Mining Industry, 2015) Кузнєцова, Олена Олександрівна
The options for improving energy efficiency of a standard residential building built in 1960s were investigated and analyzed and the structure of the heat losses of the building prior to and after the thermal renovation was determined. Thermal renovation of buildings leads to significant cost savings for space heating. Thus, the implementation of energy-efficient solutions for the analyzed building can lead to the following achievements: replacement of windows by more energy efficient ones leads to a reduction in heating energy consumption by 14,7%; insulation of external walls with bringing resistance to heat transfer to the normative values reduces heat losses by 40,4%; the most quantity of heat energy conservation (76,8%) is achieved by the integrated approach (external building envelope insulation, installation of energy efficient windows, refurbishment of the heating system). Knowing the value of investments in energy saving measures, the payback period can be calculated as well as the optimal energy-saving solution can be determined. The analysis and comparison of different energy saving solutions can be used to identify ways for further upgrading of buildings.
FEATURES OF THE MATERIAL BALANCE CALCULATION OF THREE-PHASE MIXTURES FOR EXTRACTION PROCESSES
(2018) Глибін, Віталій Ілларіонович; Кузнєцова, Олена Олександрівна
Purpose: improvement of the algorithm for calculating the liquid-liquid-vapor equilibria for binary and ternary mixtures (in application for extraction processes). Methods: analysis of phase diagrams, modeling of cutting off a part of an equilibrium phase, algebraic transformations of the system of Eqs. describing a single-step evaporation of a three-phase mixture of a given composition. Results: for two types of phase diagrams of binary mixtures with partial miscibility of components in the liquid phase, restrictions on the possible values of the mole fractions of the vapor and two liquid phases under the equilibrium conditions of the liquid-liquid-vapor mixture for a given total composition were obtained. For ternary mixtures, analytical expressions for the calculation of three phases composition for the given two sets of distribution coefficients were obtained. Formulas for calculating mole fractions of vapor and two liquid phases according to the given composition of the mixture are obtained. The following formulas are used to calculate the three-phase equilibrium of the butylene-n-butane-water mixture based on the Raoult’s and Henry’s Laws. Discussion: the obtained formulas can be used to determine approximately the composition of coexisting equilibrium phases of ternary mixtures as well as the mole fractions of phases. For binary mixtures, it is expedient to supplement the input information of the single-step evaporation of a mixture of a given composition with the permissible value of the mole fraction of the vapor phase.
Flavonoids: chemistry and biological activities
(2016) Попова, Елеонора Михайлівна; Popova, E.M.; Novza, Y.A.
In this article is described the general characteristic of flavonoids, their chemical and biological activity. The article analyses the peculiarities of flavonoids and described their importance for plant and human health.
Getting of bio- ethanol from algae Euglena viridis
(2012) Васильченко, Ольга Анатоліївна; Fedun, N.O.
The methodic of carbohydrates getting from algae Euglena viridis for bioethanol production is researched. For hydrolysis of polysaccharides was applied Anthrone method. It is determined that the content of cellulose in the culture of algae is higher than the content of starch
«Green» biotechnology: synthesis of silver nanoparticles using Artemisia tilesii «hairy» root extracts
(НФаУ, 2019-04) Ястремська, Лариса Сергіївна; Матвєєва, Н.А.; Шпетна, К.О.; Власюк, О.В.
Study the effectiveness of obtaining of silver nanoparticles using extracts from «hairy» roots of Artemisia tilesii medicinal plants was the aim of the work. The possibility of silver nanoparticles obtaining using extracts of Artemisia tilesii «hairy» root extracts was demonstrated. The most effective synthesis was found in case of using water extract. At the same time, ethanol extract also can be used for Ag nanoparticles synthesis.

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